Purification and characterization of guinea-pig chorionic gonadotrophin
نویسندگان
چکیده
منابع مشابه
Urinary chorionic gonadotrophin determination.
Successful laboratory tests for early pregnancy utilize the endogenous production and excretion of chorionic gonadotrophin (CG). There is wide variation in sensitivity and specificity of various biological and commercially available immunochemical methods for determining urinary CC levels. Normal values for immunochemically reactive CC during the first trimester of pregnancy are given. Serial d...
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Two forms of microsomal carbonyl reductase, solubilized in Triton X-100, were purified to homogeneity from the liver of male guinea pigs, primarily by affinity, DEAE-Sephacel, gel-filtration and hydroxyapatite chromatography. The major form was a tetrameric glycoprotein of single subunits of Mr 32000 and a pI value of 7.0; another minor form was a monomeric protein with Mr 34000 and a pI value ...
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The progesterone-binding plasma protein (PBP) of the pregnant guinea pig has been purified to homogeneity. The molecular weight, 77,500 (as determined by equilibrium sedimentation and sodium dodecyl sulfate polyacrylamide gel electrophoresis), the a&,,,, 4.5, the optical extinction coefficient at 280 mn E:k$mglml, 0.49, the pH<, 3.6 (isoelectrofocusing), the Stokes radius (47 A), and the partia...
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ژورنال
عنوان ژورنال: Reproduction
سال: 1984
ISSN: 1470-1626,1741-7899
DOI: 10.1530/jrf.0.0710227